RESEARCH STRICKLER LAB
DAPHNIA

One of the big technical challenges is created by the desire to see the algae entrained in the feeding current entering the carapace and getting moved up to the location in front of the mandibles (two darker half-moons left of center). We used an epi-fluorecence microscope with a blue Argon laser as light source. Natural algae will emit in the red range (auto-fluorescence). We used a near-infra-red sensitive video camera to observe the paths of the algae. Note: the algae move down to the food groove immediately without getting stuck on an appendage.

PATH OF ALGAE
This animated GIF was created by taking the odd fields of every frame, enhancing the range of gray levels, and exposing the single GIF file for 1/10 sec (instead of the video rate of 1/30 sec). Daphnia
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