Now we combine the two techniques: High speed video and epi-fluorescence microscopy. We add to the feeding current a stream of fluoresceine dye and record at 250 frames per second.

This part of our endeavor was a cooperation between Yuji Tanaka (with the help of Takashi Ishimaru who contributed the high speed video system), Klaus Plath, Matthew Brewer, Ai Nihongi, and Rudi Strickler, working over the weekend of 6 and 7 May, 2000.

Here is a ventral view into the feeding chamber with the animal's head off to the left. As the appendages move, fluoresceine is drawn into the top of the chamber. As you can see, the flow of dye through the filtering chamber is complex. We are still hours away from explaining exactly how Daphnia's feeding appendages combine to capture particles from their feeding current
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