Russell L. Cuhel -
Publications in Microbiogeochemical Ecophysiology

-- Spore Autolysins in Bacillus cereus
    (Undergraduate work)
-- Vitamins and Antarctic Cruise Measurements
    (Undergraduate work)
-- Sulfate Assimilation in Marine Bacteria
    (Graduate work)
-- Metabolism of Deep Sea Scavenging Amphipods
    (Graduate opportunity)
-- Lake Ontario Nutrient Assessment Study, 1983
    (Engaging Limnology with Canadian Centre for Inland Waters)
-- Sulfate Assimilation in Algal Cultures and Other Microbes
    (Expanding Perspectives)
-- Storm Chasing and Interdisciplinary Endeavors
    (Guest Appearances)
-- Yellowstone Lake Hydrothermal Vent Chemosynthesis
    (Life In Extreme Environments)
-- Education and Public Outreach
    ("Popular" articles)
-- Seemingly Random Research
 

Spore Autolysins in Bacillus cereus
    (Undergraduate work)

    Dr. Willie C. Brown of UCSD was the professor who turned me ON to microbiology. I had already been working with Angelo Carlucci, a marine microbiologist at Scripps Institution of Oceanography, for several years and really enjoyed the work. It was Willie whose teaching helped it all make sense. When I was on work-furlough from County Jail, Willie gave me a job for which work the following resulted. THANKS, WILLIE!!

Brown, W. C. and R. L. Cuhel. 1975. Surface-localized cortex-lytic enzyme in spores of Bacillus cereus T. J. Gen. Microbiol. 91: 429-432.

Brown, W. C., Cuhel, R. L., and C. Greer. 1977. Isolation and properties of a surface-bound, cortex-lytic enzyme from spores of Bacillus cereus T. pp. 335-349. IN: Barker, A. N., Wolf, J., Ellar, D. J., Dring, J. G., and G. W. Gould (eds.) Spore Research 1976. Academic Press, London.
 

Vitamins and Antarctic Cruise Measurements
    (Undergraduate work)

    Without any question, my enthusiasm for oceanographic research was the direct result of a dishwashing job for the Institute of Marine Research, Scripps Institution of Oceanography, UCSD. Dr. J.D.H. Strickland was in his last year before yielding to terminal illness, and three of his colleagues took me in as the "smart-ass undergraduate". They were Dr. Angelo Carlucci (microbiologist), Dr. Osmund "Ozzie" Holm-Hansen (physiologist), and Dr. Peter M. Williams (chemist). For or with these guys I gained my first 200 days at sea, worked at the CEPEX project in Saanich Inlet, and learned a tremendous array of methods and concepts. I owe them, and especially Angelo, thanks for my career. THANKS!!

Carlucci, A. F. and R. L. Cuhel. 1977. Vitamins in the South Polar Seas: distribution and significance of dissolved and particulate vitamin B12, thiamine, and biotin in the Southern Indian Ocean. pp. 115-128. IN: G. A. Llano (ed.) Adaptations Within Antarctic Ecosystems: Proceedings of the Third SCAR Symposium on Antarctic Biology. Smithsonian Institution, Washington D.C.

Holm-Hansen, O., El-Sayed, S. Z., Franceschini, G. A., and R. L. Cuhel.  1977. Primary production and the factors controlling phytoplankton  growth in the Southern Ocean. pp. 11-50. IN: G. A. Llano (ed.) Adaptations Within Antarctic Ecosystems: Proceedings of the Third SCAR Symposium on Antarctic Biology. Smithsonian Institution, Washington  D.C.
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Metabolism of Deep Sea Scavenging Amphipods
    (Graduate opportunity)

    My first ALVIN dives to Deep Ocean Station 2 off New Jersey (1700m) were thanks to Holger Jannasch's deep sea biodegradation interests of the early 1970's. I wanted to use my physiological leanings in an ecological application, and scavenging amphipods proved to be an excellent hypothesis-testing system. EVERY Oceanographer should dive in a submersible at least once!

Jannasch, H. W., Cuhel, R. L., Wirsen, C. O., and C. D. Taylor. 1980.  An approach for in situ studies of deep-sea amphipods and their microbial gut flora. Deep-Sea Res. 27: 867-872.
 

Sulfate Assimilation in Marine Bacteria
    (Graduate work)

    Using a halotolerant Pseudomonad from Galen E. Jones and a omni-lytic bacterium I isolated from a seawater toilet on the RV OCEANUS, a detailed comparative study of carbon and sulfur assimilation in marine bacteria was undertaken. My big acquisition was a thermal printer for my Texas Instruments calculator. I was provided with a sweet laboratory and office, a full stipend, and high expectations. The patience of Dr. Jannasch and the technical skills of Dr. Craig D. Taylor are greatly appreciated. If you need statistics, you've done the wrong experiment!

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1981. Assimilatory sulfur metabolism in marine microorganisms: characteristics and regulation of sulfate transport in Pseudomonas halodurans and Alteromonas luteo-violaceus. J. Bacteriol. 147: 340-349.

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1981. Assimilatory sulfur metabolism in marine microorganisms: a novel sulfate transport system in Alteromonas luteo-violaceus. J. Bacteriol. 147: 350-353.

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1981. Assimilatory sulfur metabolism in marine microorganisms: sulfur metabolism, growth, and protein synthesis of Pseudomonas halodurans and Alteromonas luteo-violaceus during sulfate limitation. Arch. Microbiol. 130: 1-7.

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1981. Assimilatory sulfur metabolism in marine microorganisms: sulfur metabolism, protein synthesis, and growth of Pseudomonas halodurans and Alteromonas luteo-violaceus during unperturbed batch growth. Arch. Microbiol. 130: 8- 13.

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1982. Assimilatory sulfur metabolism in marine microorganisms: sulfur metabolism, protein synthesis, and growth of Pseudomonas halodurans and Alteromonas luteo-violaceus during perturbed batch growth. Appl. Environ. Microbiol. 43: 151-159.

Cuhel, R. L., Taylor, C. D., and H. W. Jannasch. 1982. Assimilatory sulfur metabolism in marine microorganisms: considerations for the application of sulfate incorporation into protein as a measurement of natural population protein synthesis. Appl. Environ. Microbiol. 43: 160-168.

Cuhel, R. L., Jannasch, H. W., Taylor, C. D., and D. R. S. Lean. 1983. Microbial growth and macromolecular synthesis in the northwestern Atlantic Ocean. Limnol. Oceanogr. 28: 1-18.
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Sulfate Assimilation in Algal Cultures and Other Microbes
    (Expanding Perspectives)

    Inorganic nutrient assimilation is a characteristic exclusive to the microbes (bacteria, algae/plants, and fungi). Monumentally different ecophysiological behaviors occur among these broad groups as well as within them. These and many other, as yet unpublished forays into diverse microbial physiological mechanisms has been much, much more than exciting.

Cuhel, R. L. and J. B. Waterbury. 1984. Biochemical composition and short- term nutrient incorporation patterns in a unicellular marine Cyanobacterium, Synechococcus sp. Limnol. Oceanogr. 29: 370-374.

Cuhel, R. L., Ortner, P. B., and D. R. S. Lean. 1984. Night synthesis of protein by algae. Limnol. Oceanogr. 29: 731-744.

Pick, F. R. and R. L. Cuhel. 1986. Light quality effects on carbon and sulfur uptake of a metalimnetic population of the colonial chrysophyte Chrysosphaerella longispina. pp. 197-205 IN: Kristiansen, J. and R. Anderson [eds.] Chrysophytes: Aspects and Problems. Cambridge University Press, New York.
 

Lake Ontario Nutrient Assessment Study, 1983
    (Engaging Limnology with Canadian Centre for Inland Waters)

    Dr. David R.S. Lean from the Canadian Centre for Inland Waters approached me after my "dissertation talk" at an American Society of Limnology and Oceanography meeting. He wanted to see how microbial sulfate assimilation might be applied to lake productivity measurements. During the 14 cruises and associated lab work at CCIW during 1983, we together applied subcellular techniques to a wide range of nutrient uptake experiments. This effort developed capabilities in both algal physiology and freshwater applications, for which I am grateful. THANKS, DAVE!

Cuhel, R. L. and D. R. S. Lean. 1987. Protein synthesis by lake plankton measured using in situ carbon and sulfur assimilation. Can. J. Fish. Aquat. Sci. 44: 2102-2117.

Cuhel, R. L. and D. R. S. Lean. 1987. Influence of light intensity, light quality, temperature, and daylength on uptake and assimilation of carbon dioxide and sulfate by lake plankton. Can. J. Fish. Aquat. Sci. 44: 2118-2132.

Lean, D. R. S. and R. L. Cuhel. 1987. Subcellular phosphorus kinetics for Lake Ontario plankton. Can. J. Fish. Aquat. Sci. 44: 2077-2086.

Lean, D. R. S., H. J. Fricker, M. N. Charlton, R. L. Cuhel, and F. R. Pick. 1987. The Lake Ontario life support system. Can. J. Fish. Aquat. Sci. 44: 2230-2240.

Putt, M., Harris, G. P., and R. L. Cuhel. 1987. Photoinhibition of DCMU- enhanced fluorescence in Lake Ontario phytoplankton. Can. J. Fish. Aquat. Sci. 44: 2144-2154.

Lean, D. R. S., Cuhel, R. L., and M. N. Charlton. 1989. Protein synthesis: a measure of growth for lake plankton. Hydrobiologia 173: 119-126.
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Storm Chasing and Interdisciplinary Endeavors
    (Guest Appearances)

    Ever since my first cruise from Australia to the Ross Ice Shelf, Antarctica I have been interested in physical-biological coupling in aquatic ecosystems. Many efforts in this arena, well represented in the Meeting Abstracts section, have been accomplished largely because I don't get seasick under any conditions. This led to invitations to collaborate on interdisciplinary cruises specifically focused on some aspect of storm influences on ecosystem behavior. In fact, Carmen and I were married on the RV CAPE HATTERAS during Hurricane Marilyn on one of Fogel and Paerl's atmospheric deposition study cruises!

Bates, T. S., Kiene, R. P., Wolfe, G. V., Matrai, P. M., Chavez, F. P., Buck, K. R., Blomquist, B. W., and R. L. Cuhel. 1994. The cycling of sulfur in surface seawater of the northeast Pacific. J. Geophys. Res. 99(C4): 7835-7843.

Fogel, M.L., Aguilar, C., Cuhel, R.L., Hollander, D.J., Willey, J.D., and H.W. Paerl. 1999. Biological and isotopic changes in coastal waters induced by Hurricane Gordon.  Limnol. Oceanogr. 44: 1359-1369.
 

Yellowstone Lake Hydrothermal Vent Chemosynthesis
    (Life In Extreme Environments)

    Yellowstone Lake is a continental hot-spot with active geothermal processes. The sub-aerial ("under air") boiling pots and mud volcanoes have been extensively studied and yielded phenomenal scientific and economic (TAQ polymerase) benefits. The underwater vents pose a more paleo- and exo-biological opportunity, possibly resembling early life on Earth or that possible on other planets (Mars polar cap, Europa). My first trip to make measurements of chemosynthesis was in 1994 when water levels were exceptionally low and technological capabilities were still primitive. Tremendous dark carbon dioxide fixation was found, even in surface waters. Gearing up with HPLC for sulfur analysis and a much more elaborate rate measurement set-up, a developing characterization shows Yellowstone Lake vents to be a vigorous, exciting microbiogeochemical ecosystem. See also Meeting Abstracts.

Buchholz, L. A., Anderson, P. D., Cuhel, R. L., Klump, J. V., Kostka, J. E., Paddock, R. W., Remsen, C. C., Maki, J. S., and Lovalvo, D. 1995. Employment of ROV techniques and Scuba in Yellowstone Lake. IN: D.E.J. Harper (ed.), Diving for Science: Nahant, MA, American Academy of Underwater Science, p. 1-7.

Cuhel, R.L., Aguilar, C., Anderson, P.D., Maki, J.S., Paddock, R.W., Remsen, C.C., Klump, J.V., and D. Lovalvo. 2002. Underwater domains in Yellowstone Lake hydrothermal vent geochemistry and bacterial chemosynthesis. pp. 27-53. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

Cuhel, R.L., Aguilar, C., Remsen, C.C., Maki, J.S., Lovalvo, D., Klump, J.V., and R.W. Paddock. 2002. The Bridge Bay spires: Collection and preparation of a scientific specimen and museum piece. pp. 54-68. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

Aguilar, C., Cuhel, R.L., and J.V. Klump. 2002. Porewater and hydrothermal vent water inputs to Yellowstone Lake, Wyoming. pp. 1-18. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

Klump, J.V., Kaster, J., Fitzgerald, S., Remsen, C.C., Anderson, P., Paddock, R.W., Aguilar, C., Cuhel, R.L., Maki, J.S., and D. Lovalvo. 2002. Climate, Tectonics, or ?: Speculations on the Recent Paleolimnology of Yellowstone Lake. pp. 89-100. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

Maki, J.S., Schroeder, C.M., Bruckner, J.C., Wimpee, C., Weir, A., Remsen, C.C., Aguilar, C., and R.L. Cuhel. 2002. Investigating the microbial ecology of Yellowstone Lake. pp. 101-113. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

Remsen, C.C., Maki, J.S., Klump, J.V., Aguilar, C., Anderson, P.D., Buchholz, L., Cuhel, R.L., Lovalvo, D., Paddock, R.W., Waples, J.T., Bruckner, J.C., and C.M. Schroeder. 2002. Sublacustrine Geothermal Activity in Yellowstone Lake: A Summary of 17 Years of Research. pp. 192-212. IN: Anderson, R.J. and D. Harmon (eds.), Yellowstone Lake: Hotbed of Chaos or Reservoir of Resilience? Proceedings of the 6th Biennial Conference on the Greater Yellowstone Ecosystem. October 8-10, 2001, Mammoth Hot Springs Hotel,Yellowstone National Park. Yellowstone National Park, WY and Hancock, MI: Yellowstone Center for Resources and The George Wright Society.

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Seemingly Random Research

Henrichs, S. M. and R. L. Cuhel. 1985. Occurrence of  b-aminoglutaric acid in marine bacteria. Appl. Environ. Microbiol. 50: 543-545.

Cuhel, R. L. 1993. Photoassimilation of acetate by algae. pp. 433-440 IN: Kemp, P. F., Sherr, B. F., Sherr, E. B., and J. J. Cole (eds.). Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton.

Cuhel, R. L. 1993. Sulfate assimilation by aquatic microorganisms. pp. 611-620 IN: Kemp, P. F., Sherr, B. F., Sherr, E. B., and J. J. Cole (eds.). Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton.

Mayer, P., Cuhel, R. L., and N. Nyholm. 1997. A simple in vitro fluorescence method for biomass measurements in algal growth inhibition tests.  Water. Res. 31(10): 2525-2531.
 

Education and Public Outreach
    ("Popular" articles)

    It was only when I arrived at the UWM Center for Great Lakes Studies that opportunities to practice broadcasting research to the public arose. I love this aspect of our work. It started with deep integration of student interns in active research activities (especially Elkhart Lake research) and expanded into teacher training, Earth Day and a wide variety of other public venues. The "Research Experience for Undergraduates" site at CGLS provided a basis from which to leap. Taken over from its initiators (Nealson, Remsen, and Sommer) in its second year (1990), the REU has expanded into a broad and highly integrated aquatic science education effort.

Klump, J.V., Remsen, C.C., Lovalvo, D., Anderson, P., Cuhel, R.L., Kaplinski, M. Kaster, J., Maki, J., and R. Paddock. 1995. 20,000 leagues under Yellowstone Lake.  Yellowstone Sci. 3(4): 10-14.

Cuhel, R.L. and C. Aguilar. 1998. Research Experience for Undergraduates in Aquatic Sciences: Students gain early career access at ASLO 99 - Santa Fe. ASLO Bulletin 7(3): 16-17.

Cuhel, R.L. 2000. Shipboard research experience for undergraduates: Building skills, self-confidence, and leadership potential. ASLO Bulletin 9(2): 22-23.

Cuhel, R.L., Aguilar, C., Remsen, C.C., Maki, J.S., Lovalvo, D., Klump, J.V., and R.W. Paddock. (2002). The Bridge Bay spires: Collection and preparation of a scientific specimen and museum piece. Yellowstone Science ??: PPP-PPP.
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